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Platform
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Illumina MiSeq
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Ion Torrent PGM
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PacBio RS
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Illumina GAIIx
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Illumina HiSeq 2000
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|---|
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Instrument Cost*
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$128 K
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$80 K**
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$695 K
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$256 K
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$654 K
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|
Sequence yield per run
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1.5-2Gb
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20-50 Mb on 314 chip, 100-200 Mb on 316 chip, 1Gb on 318 chip
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100 Mb
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30Gb
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600Gb
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Sequencing cost per Gb*
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$502
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$1000 (318 chip)
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$2000
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$148
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$41
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Run Time
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27 hours***
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2 hours
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2 hours
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10 days
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11 days
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Reported Accuracy
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Mostly > Q30
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Mostly Q20
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<Q10
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Mostly > Q30
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Mostly > Q30
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Observed Raw Error Rate
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0.80 %
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1.71 %
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12.86 %
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0.76 %
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0.26 %
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Read length
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up to 150 bases
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~200 bases
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Average 1500 bases**** (C1 chemistry)
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up to 150 bases
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up to 150 bases
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Paired reads
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Yes
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Yes
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No
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Yes
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Yes
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Insert size
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up to 700 bases
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up to 250 bases
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up to 10 kb
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up to 700 bases
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up to 700 bases
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Typical DNA requirements
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50-1000 ng
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100-1000 ng
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~1 μg
|
50-1000 ng
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50-1000 ng
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- * All cost calculations are based on list price quotations obtained from the manufacturer and assume expected sequence yield stated.
- ** System price including PGM, server, OneTouch and OneTouch ES.
- *** Includes two hours of cluster generation.
- **** Mean mapped read length includes adapter and reverse strand sequences. Subread lengths, i.e. the individual stretches of sequence originating from the sequenced fragment, are significantly shorter.
